Recently, the functional genome team of the Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, and Beijing Weixinyu Biotech Co., Ltd. jointly sponsored a special project of the Ministry of Science and Technology's innovation project. “The new method of Raman optical forceps screening in live cell single-cell high-throughput separation The application "has passed the review and acceptance, which marks the successful development of the world's first living single-cell Raman separator in China.

The research was completed under the auspices of Xu Jian and a part-time researcher of the Qingdao Energy Institute and Huang Biao of the University of Sheffield in the United Kingdom. The project team's research and development is the first cell manual and automatic sorting instrument based on cell Raman fingerprinting. The sorter can achieve rapid single-cell Raman spectra collection, and the first time the single-cell Raman signal acquisition time shortened to 1 to 100 milliseconds; can also complete Raman map-based cell types and growth status of rapid identification and many other task.

The core advantage of this instrument is that it is sensitive to cell biochemical information and its changes. There is no need to anticipate biomarkers or label cells, and in situ and non-invasive bioassays can be performed. This technology will make a positive contribution to the research of single cell biotechnology and single cell genomes.

The project team has used this instrument to obtain preliminary results in the study of the physiological status of photosynthetic oil-producing microalgae and the separation of PAH-degrading microorganisms, and established the reference methods and data analysis procedures for applied demonstration technologies.

It is understood that the instrument currently serves a number of research teams at home and abroad, including marine resource excavation, biofuels and biomaterials, bioenergy germplasm screening, food microbiological testing, drug research, cancer monitoring and sorting, and environmental microbiological monitoring. Agricultural ecological research and other fields play an important role.